                         SEQUENCE LISTING

<110>  Smith, Hilde
       Ingebritson, Alaina
       Neubauer, Axel
       de Greeff, Astrid
 
<120>  Attenuating bacterial virulence by attenuating bacterial folate 
       transport

<130>  3811-P111017US

<160>  27    

<170>  PatentIn version 3.5

<210>  1
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer comE1

<400>  1
cgagctcgga agaattggtt attgcgcgtg                                        30


<210>  2
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer comE3

<400>  2
cgggatcccg ggggatgacc tgttgcttg                                         29


<210>  3
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer comE3

<400>  3
tcccccgggg gagtcgtgtg tattcgacag cgg                                    33


<210>  4
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer comE4

<400>  4
tcccccgggg gacaagcaac aggtcatccc c                                      31


<210>  5
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer comE6

<400>  5
cgggatcccg gttgaatgcc cggcaagcc                                         29


<210>  6
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Orf2-fw

<400>  6
ctacggctgg ttcttctatc gaa                                               23


<210>  7
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Orf2-rev

<400>  7
gcaatcggtg tcatgataaa gg                                                22


<210>  8
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer folC-fw

<400>  8
gtttgtccgt ccatcggttt                                                   20


<210>  9
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer folC-rev

<400>  9
ctggtcggtc gcatagatga                                                   20


<210>  10
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer RecA-fw

<400>  10
ggtttgcagg ctcgtatgat g                                                 21


<210>  11
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer RecA-rev

<400>  11
accaaacatg acaccgactt ttt                                               23


<210>  12
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer t488a

<400>  12
gaaaggtata gtttttagca agtggacaaa atatatagtg tgtgatacaa t                51


<210>  13
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer t488a_antisense

<400>  13
attgtatcac acactatata ttttgtccac ttgctaaaaa ctataccttt c                51


<210>  14
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer V735-fw

<400>  14
tatgcgcaat gacgtagtag aagg                                              24


<210>  15
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer M13-rev

<400>  15
aacagctatg accatg                                                       16


<210>  16
<211>  5
<212>  PRT
<213>  Streptococcus suis

<400>  16

Phe Tyr Arg Lys Pro 
1               5   


<210>  17
<211>  168
<212>  PRT
<213>  Clostridium bolteae

<400>  17

Met Thr Lys Thr Lys His Met Val Trp Met Gly Ile Leu Ile Ala Val 
1               5                   10                  15      


Ser Ile Val Leu Ser Arg Phe Leu Ser Phe Ser Ala Trp Asn Val Lys 
            20                  25                  30          


Ile Gly Phe Ala Phe Ile Pro Ile Val Ile Gly Ala Val Leu Phe Gly 
        35                  40                  45              


Pro Val Gln Gly Gly Ile Ala Ala Ala Ala Ala Asp Phe Leu Gly Ala 
    50                  55                  60                  


Ile Leu Phe Pro Ile Gly Met Tyr Phe Pro Gly Phe Thr Val Thr Ala 
65                  70                  75                  80  


Phe Leu Thr Gly Leu Thr Tyr Gly Ile Leu Leu His Lys Asn Arg Ser 
                85                  90                  95      


Met Phe Arg Ile Ala Cys Ala Val Leu Ile Val Gln Leu Val Tyr Gly 
            100                 105                 110         


Leu Leu Leu Asn Thr Cys Trp Ile Ser Leu Leu Tyr Gly Ala Pro Tyr 
        115                 120                 125             


Leu Ala Leu Leu Ser Thr Arg Ile Val Gln Tyr Val Val Leu Ile Pro 
    130                 135                 140                 


Val Gln Phe Val Ile Ile Ala Arg Met Tyr Val Leu Gly Ser Lys Lys 
145                 150                 155                 160 


Tyr His Ile Leu Gln Glu Asn Ser 
                165             


<210>  18
<211>  187
<212>  PRT
<213>  Clostridium phytofermentans

<400>  18

Met Leu Asn Gln Glu Lys Asn Val Lys Asn Lys Asp Leu Lys Lys Gly 
1               5                   10                  15      


Lys Lys Val Phe Thr Leu Glu Thr Phe Ile Val Leu Ala Leu Leu Val 
            20                  25                  30          


Ala Ile Glu Val Ile Leu Thr Arg Phe Leu Ser Leu Lys Glu Trp Asn 
        35                  40                  45              


Ile Arg Phe Ser Phe Gly Phe Ile Pro Val Val Ile Ala Ala Ile Leu 
    50                  55                  60                  


Tyr Gly Pro Ile Ala Ser Ala Thr Val Ala Ala Cys Ser Asp Phe Leu 
65                  70                  75                  80  


Gly Ala Ile Leu Phe Pro Met Gly Ala Tyr Phe Pro Gly Phe Thr Ile 
                85                  90                  95      


Thr Ala Phe Ile Ser Gly Ile Val Tyr Gly Leu Phe Leu His Lys Lys 
            100                 105                 110         


Gln Ser Leu Pro Asn Ile Val Gly Ala Ala Val Val Asn Gln Phe Phe 
        115                 120                 125             


Cys Gly Leu Val Ile Asn Ser Tyr Trp Leu Ser Ile Ile Ser Gly Lys 
    130                 135                 140                 


Ser Thr Phe Trp Gly Leu Ile Pro Ile Arg Ser Ile Gln Ser Ala Val 
145                 150                 155                 160 


Met Ser Ile Val Ile Ile Ser Val Thr Tyr Val Ile Ser Lys Thr Ile 
                165                 170                 175     


Val Pro Ile Ile Lys Lys Ala Ile Val Ile Met 
            180                 185         


<210>  19
<211>  189
<212>  PRT
<213>  Alkaliphilus metalliredigens

<400>  19

Met Lys Phe Asn Thr Arg Lys Leu Val Thr Leu Ser Leu Leu Met Ala 
1               5                   10                  15      


Leu Thr Ile Val Phe Thr Arg Ile Ala Ser Ile Arg Ile Pro Phe Gly 
            20                  25                  30          


Gly Val Glu Gly Val Arg Val Gly Phe Gly Ser Leu Pro Ile Leu Leu 
        35                  40                  45              


Ala Gly Ile Leu Phe Gly Pro Ile Ser Gly Phe Ile Val Gly Ala Leu 
    50                  55                  60                  


Gly Asp Leu Ile Gly Tyr Phe Leu Asn Pro Met Gly Ala Tyr Met Pro 
65                  70                  75                  80  


His Phe Thr Leu Ser Ala Gly Leu Ser Gly Phe Ile Pro Gly Ser Ile 
                85                  90                  95      


Tyr Tyr Phe Thr Phe Arg Pro Lys Ser Asn Ile His Phe Ser Ser Lys 
            100                 105                 110         


Leu Gln Val Ser Arg Pro Ser Phe Trp Leu Ile Phe Ile Ser Ile Leu 
        115                 120                 125             


Ile Gly Gln Val Thr Ile Ser Leu Leu Leu Ile Pro Tyr Phe Leu Ser 
    130                 135                 140                 


Ala Leu Phe Ser Ile Pro Tyr Glu Leu Thr Ile Ile Pro Arg Thr Ile 
145                 150                 155                 160 


Thr Gln Leu Ile Ser Ile Pro Ile Phe Ser Trp Val Ile Trp Ile Ile 
                165                 170                 175     


Ser Asn Lys Thr Asn Ile Phe Asp Tyr Val Lys Ser Lys 
            180                 185                 


<210>  20
<211>  162
<212>  PRT
<213>  Thermoanaerobacter tengcongensis

<400>  20

Met Lys Lys Phe Thr Thr Arg Glu Ile Ala Phe Leu Ala Leu Leu Val 
1               5                   10                  15      


Ala Leu Asn Ile Val Leu Thr Arg Ile Ala Ser Ile Arg Ile Ala Ile 
            20                  25                  30          


Gly Gly Val Glu Gly Ile Arg Ile Gly Phe Gly Ala Phe Pro Val Ile 
        35                  40                  45              


Phe Ser Gly Ile Ala Phe Gly Pro Tyr Ala Gly Gly Ile Val Gly Ala 
    50                  55                  60                  


Leu Gly Asp Ile Ile Gly Tyr Phe Ile Asn Pro Met Gly Pro Tyr Met 
65                  70                  75                  80  


Pro His Phe Thr Phe Thr Ala Ala Leu Val Gly Ile Leu Pro Pro Leu 
                85                  90                  95      


Phe Leu Lys Pro Phe Lys Ala Gln Ile Pro Thr Phe Trp Gln Leu Val 
            100                 105                 110         


Ile Ala Ile Gly Leu Gly Gln Thr Ile Ser Ser Ile Ile Leu Thr Pro 
        115                 120                 125             


Tyr Phe Ile Gln Met Leu Phe His Leu Pro Met Lys Ile Thr Val Pro 
    130                 135                 140                 


Pro Arg Ile Val Thr Gln Ala Ile Gln Val Pro Leu Tyr Ala Phe Leu 
145                 150                 155                 160 


Leu Lys 
        


<210>  21
<211>  175
<212>  PRT
<213>  Enterococcus faecium

<400>  21

His Arg Leu Asp Ala Arg Met Ile Ala Ile Met Gly Leu Leu Ile Ala 
1               5                   10                  15      


Leu Met Val Thr Leu Ser Arg Leu Val Ala Ile Glu Thr Pro Phe Ile 
            20                  25                  30          


Lys Ile Ser Val Thr Phe Ile Pro Gln Val Ile Met Gly Ile Leu Phe 
        35                  40                  45              


Gly Pro Phe Trp Ser Gly Ile Gly Ala Val Leu Ala Asp Leu Val Gly 
    50                  55                  60                  


Met Ala Leu Phe Ser Lys Ser Ala Phe Phe Ile Gly Phe Thr Leu Asn 
65                  70                  75                  80  


Ala Phe Ile Glu Gly Ala Ile Tyr Gly Phe Phe Phe Tyr Arg Lys Glu 
                85                  90                  95      


Ile Thr Trp Lys Asn Ala Ile Leu Ala Thr Leu Ser Val Thr Leu Ile 
            100                 105                 110         


Ile Asn Leu Phe Leu Thr Pro Leu Trp Leu Ala Leu Met Tyr His Val 
        115                 120                 125             


Pro Leu Phe Ser Trp Val Val Trp Ala Pro Arg Leu Leu Lys Thr Val 
    130                 135                 140                 


Ile Trp Leu Pro Ile Gln Ser Ile Ala Ile Tyr Tyr Val Gly Arg Ser 
145                 150                 155                 160 


Ile Pro Tyr Lys Lys Ile Leu Arg Ser Leu Ala Ile His Ala Lys 
                165                 170                 175 


<210>  22
<211>  179
<212>  PRT
<213>  Enterococcus faecalis

<400>  22

Met Thr Lys Lys Lys Phe Gly Thr Lys Ser Ile Ala Leu Met Gly Val 
1               5                   10                  15      


Leu Ile Ala Val Val Val Val Phe Ser Arg Phe Phe Ala Tyr Glu Thr 
            20                  25                  30          


Thr Phe Leu Lys Ile Ser Phe Thr Phe Ile Pro Glu Ser Leu Ile Gly 
        35                  40                  45              


Met Ile Phe Gly Pro Phe Trp Ala Gly Ile Gly Thr Ala Val Ala Asp 
    50                  55                  60                  


Val Val Gly Met Leu Leu Phe Pro Lys Ala Gly Tyr Phe Pro Gly Phe 
65                  70                  75                  80  


Thr Leu Asn Ala Phe Leu Ala Gly Ala Ile Tyr Gly Tyr Phe Tyr Tyr 
                85                  90                  95      


Lys Lys Glu Met Thr Trp Gln Arg Val Ile Leu Ala Thr Leu Leu Val 
            100                 105                 110         


Thr Val Leu Ile Asn Ile Ile Leu Thr Pro Leu Trp Leu Ser Leu Met 
        115                 120                 125             


Tyr Gly Val Asn Leu Ala Asn Phe Ala Trp Trp Val Pro Arg Leu Ile 
    130                 135                 140                 


Lys Thr Val Ile Phe Phe Pro Ile Gln Val Ile Ala Thr Tyr Tyr Leu 
145                 150                 155                 160 


Gly Asn Lys Ile Pro Phe Lys Arg Leu Phe Gly Lys Pro Leu Ser Glu 
                165                 170                 175     


Leu Asp Gln 
            


<210>  23
<211>  177
<212>  PRT
<213>  Lactobacillus brevis

<400>  23

Met Lys Thr Met Ala Lys Thr Gln Leu Pro Lys Leu Asp Thr Leu Ser 
1               5                   10                  15      


Met Val Thr Met Gly Val Leu Met Ala Leu Gln Leu Val Ile Ser Arg 
            20                  25                  30          


Phe Ser Val Gly Asn Asn Phe Ile Lys Val Ser Phe Thr Phe Leu Ile 
        35                  40                  45              


Val Ala Leu Ile Ala Lys Trp Phe Gly Pro Trp Trp Gly Met Leu Thr 
    50                  55                  60                  


Ala Ala Val Val Asp Val Ile Gly Thr Leu Met Thr Gly Gly Pro Phe 
65                  70                  75                  80  


Phe Ile Gly Phe Thr Val Ser Ala Val Leu Gly Ser Leu Ile Tyr Ala 
                85                  90                  95      


Val Phe Leu Tyr Arg Gln Pro Val Ser Trp Trp Arg Val Ile Gly Ala 
            100                 105                 110         


Ser Val Leu Ile Ala Leu Leu Val Asn Thr Leu Leu Asn Thr Leu Trp 
        115                 120                 125             


Val Thr Ile Met Tyr Gln Thr Pro Phe Trp Ser Leu Leu Pro Val Arg 
    130                 135                 140                 


Ala Leu Lys Glu Leu Ile Val Thr Pro Val Gln Ile Val Leu Val Tyr 
145                 150                 155                 160 


Leu Leu Leu Lys Ser Gln Val Ile Gln Met Ile Gln Ala Arg Leu Asn 
                165                 170                 175     


Lys 
    


<210>  24
<211>  186
<212>  PRT
<213>  Streptococcus mutans

<400>  24

Met Asn Thr Met Phe Lys Ser Pro Lys Leu Ser Pro Gln Arg Leu Val 
1               5                   10                  15      


Thr Leu Ala Met Leu Ile Ala Leu Ala Phe Ala Ile Gly Lys Phe Ser 
            20                  25                  30          


Ile Pro Ile Ile Pro Gln Gln Leu Ile Ile Ser Pro Thr Phe Ile Val 
        35                  40                  45              


Asn Val Met Ile Gly Met Ile Gly Gly Pro Ile Trp Ala Phe Ile Ser 
    50                  55                  60                  


Leu Ala Ile Leu Asp Ile Val Asp Asn Leu Ser Ser Gly Ala Gly Asn 
65                  70                  75                  80  


Phe Ile Ile Trp Trp Thr Leu Leu Glu Ala Val Gln Gly Leu Phe Tyr 
                85                  90                  95      


Gly Leu Phe Phe Tyr Gln Lys Ser Leu Ser Trp Thr Asn Lys Lys Asp 
            100                 105                 110         


Trp Leu His Val Thr Ile Ala Thr Ala Ile Ile Met Leu Ile Gly Ser 
        115                 120                 125             


Phe Ile Phe Thr Pro Leu Leu Val Gln Ile Tyr Tyr Gly Val Pro Phe 
    130                 135                 140                 


Trp Ala Gln Phe Ala Ala Gly Arg Trp Leu Lys Ile Phe Glu Ile Pro 
145                 150                 155                 160 


Ile Arg Ile Leu Val Thr Met Ala Ile Met Pro Gln Leu Gln Arg Ile 
                165                 170                 175     


Pro Glu Leu Arg Lys Leu Ala Asn Phe Lys 
            180                 185     


<210>  25
<211>  186
<212>  PRT
<213>  Streptococcus gallolyticus

<400>  25

Met Asn Leu Phe Phe Lys Thr Pro Lys Leu Thr Leu Lys Arg Leu Val 
1               5                   10                  15      


Ser Leu Ala Met Leu Ile Ala Leu Ala Phe Ile Val Gly Lys Phe Ser 
            20                  25                  30          


Ile Pro Val Ile Pro Gln Gln Leu Val Val Ser Leu Thr Phe Ile Val 
        35                  40                  45              


Asn Thr Ile Ile Gly Met Ile Gly Gly Pro Ile Trp Gly Phe Ile Ser 
    50                  55                  60                  


Leu Gly Ile Leu Asp Val Val Asp Thr Leu Ser Ser Ser Ser Ala Gly 
65                  70                  75                  80  


Asn Phe Ile Ile Trp Trp Thr Leu Met Glu Ala Ile Gln Gly Phe Phe 
                85                  90                  95      


Tyr Gly Leu Phe Phe Tyr Gly Lys Pro Leu Ser Trp Ser Ser Lys Lys 
            100                 105                 110         


Asp Trp Leu His Val Thr Ile Ala Thr Val Val Ile Met Leu Ile Gly 
        115                 120                 125             


Thr Phe Ile Leu Thr Pro Leu Leu Ile Gln Ile Tyr Phe Gly Val Pro 
    130                 135                 140                 


Phe Trp Ala Gln Tyr Leu Ala Gly Arg Trp Leu Lys Ile Phe Glu Ile 
145                 150                 155                 160 


Pro Leu Arg Ile Ile Ile Thr Met Leu Val Ile Pro Arg Leu Gln Lys 
                165                 170                 175     


Ile Pro Glu Leu Arg Lys Leu Ala Asn Leu 
            180                 185     


<210>  26
<211>  185
<212>  PRT
<213>  Streptococcus uberis

<400>  26

Met Pro Lys Gln Leu Tyr Phe Pro Lys Leu Thr Val Gln Arg Leu Val 
1               5                   10                  15      


Thr Leu Ala Met Leu Ile Ala Leu Ala Val Ile Val Ser Lys Phe Ser 
            20                  25                  30          


Val Ser Ile Ile Pro Asn Gln Leu Val Ile Ser Phe Thr Phe Ile Val 
        35                  40                  45              


Asn Thr Val Ile Gly Ile Ile Ala Gly Pro Phe Trp Ser Phe Ile Thr 
    50                  55                  60                  


Leu Ala Met Ile Asp Leu Ile Asp Ser Leu Met Gly Gly Thr Ser His 
65                  70                  75                  80  


Phe Ile Ile Trp Trp Thr Val Met Glu Ala Phe Gln Gly Leu Leu Tyr 
                85                  90                  95      


Gly Phe Phe Phe Tyr Lys Arg Pro Leu Arg Ser Asn Gln Lys Lys Asp 
            100                 105                 110         


Trp Ile Tyr Val Ser Ala Val Thr Leu Val Ile Met Leu Phe Ser Thr 
        115                 120                 125             


Phe Leu Ile Thr Pro Leu Leu Ile Gln Ile Tyr Phe His Val Pro Phe 
    130                 135                 140                 


Trp Ala Gln Tyr Ala Ala Gly Arg Trp Phe Lys Ile Phe Glu Ile Pro 
145                 150                 155                 160 


Leu Arg Val Leu Leu Thr Met Phe Leu Ile Pro Pro Leu Gln Arg Ile 
                165                 170                 175     


Pro Glu Ile Lys Lys Leu Ser Ala Leu 
            180                 185 


<210>  27
<211>  183
<212>  PRT
<213>  Streptococcus suis

<400>  27

Met Glu Lys Lys Ile Pro Lys Leu Thr Val Gln Leu Leu Ala Ala Ile 
1               5                   10                  15      


Ala Met Thr Leu Ala Leu Val Met Ile Val Glu Asn Tyr Phe Ser Ile 
            20                  25                  30          


Arg Ile Ser Asp Thr Leu Gln Val Gln Phe Thr Phe Ile Pro Asn Thr 
        35                  40                  45              


Ile Leu Gly Ala Ile Ala Gly Pro Val Trp Ala Ala Val Phe Ala Ala 
    50                  55                  60                  


Ile Ser Asp Pro Val Phe Val Leu Phe Ser Gly Gln Thr Val Leu Phe 
65                  70                  75                  80  


Thr Trp Ile Leu Ile Glu Ala Val Ser Ala Phe Ile Tyr Gly Trp Phe 
                85                  90                  95      


Phe Tyr Arg Lys Pro Leu Asp Thr Lys Asn Lys Ala Asp Trp Leu Tyr 
            100                 105                 110         


Val Ala Gly Val Val Val Leu Ile Gln Val Val Ile Ser Phe Ile Met 
        115                 120                 125             


Thr Pro Ile Ala Leu His Phe His Phe Gly Thr Pro Trp Ile Val Leu 
    130                 135                 140                 


Tyr Ser Ser Arg Leu Ile Lys Ala Val Phe Glu Ile Pro Leu Arg Ile 
145                 150                 155                 160 


Val Val Thr Met Leu Val Leu Pro Ser Leu Gln Lys Ile Pro Glu Leu 
                165                 170                 175     


Ala Lys Leu Met Gly Ile Lys 
            180             


